Polytene chromosomes from ovarian nurse cells of Drosophila melanogaster otu mutants.
نویسندگان
چکیده
Preface ..............................................................................................................v Contributors ..................................................................................................... ix Color Plates ................................................................................................... xiii 1. The Chromosomes of Drosophila melanogaster Daryl S. Henderson ............................................................................... 1 2. Spermatogenesis: Analysis of Meiosis and Morphogenesis Helen White-Cooper ........................................................................... 45 3. Time-Lapse Imaging of Male Meiosis by Phase-Contrast and Fluorescence Microscopy Elena Rebollo and Cayetano González ............................................... 77 4. Immunocytological Analysis of Oogenesis Endre Máthé ........................................................................................ 89 5. Cytological Analysis of Oogenesis Seppo Nokkala and Christina Nokkala ............................................. 129 6. Polytene Chromosomes From Ovarian Nurse Cells of Drosophila melanogaster otu Mutants Dmitry E. Koryakov, Natalia I. Mal’ceva, Robert C. King, and Igor F. Zhimulev .................................................................... 139 7. Feulgen–DNA Cytophotometry for Estimating C Values Ellen M. Rasch ................................................................................... 163 8. Fluorescent BrdU Labeling and Nuclear Flow Sorting of the Drosophila Ovary Brian R. Calvi and Mary A. Lilly ....................................................... 203 9. Studying Nuclear Organization in Embryos Using Antibody Tools Kristen M. Johansen and Jørgen Johansen ........................................ 215 10. Whole-Mount Fluorescence In Situ Hybridization to Chromosomes of Embryos Daryl S. Henderson ........................................................................... 235 11. Orcein Staining and the Identification of Polytene Chromosomes John Tonzetich .................................................................................. 249 12. Salivary Chromosome Analysis of Aberrations Adelaide T. C. Carpenter .................................................................. 257 13. In Situ Hybridization to Polytene Chromosomes Robert D. C. Saunders ...................................................................... 279
منابع مشابه
Hrb27C, Sqd and Otu cooperatively regulate gurken RNA localization and mediate nurse cell chromosome dispersion in Drosophila oogenesis.
Heterogeneous nuclear ribonucleoproteins, hnRNPs, are RNA-binding proteins that play crucial roles in controlling gene expression. In Drosophila oogenesis, the hnRNP Squid (Sqd) functions in the localization and translational regulation of gurken (grk) mRNA. We show that Sqd interacts with Hrb27C, an hnRNP previously implicated in splicing. Like sqd, hrb27C mutants lay eggs with dorsoventral de...
متن کاملAn ultrastructural study of ovarian development in the otu7 mutant of Drosophila melanogaster.
Females homozygous for the otu7 allele produce ovarian tumours, as well as egg chambers that reach a relatively late stage of development. Mutant ovarian nurse cells contain giant polytene chromosomes. These are transcriptionally active, and RNA is transported to the oocyte through ring canals, although at reduced rate. Vitellogenic oocytes are endocytotically active. Protein (alpha yolk) spher...
متن کاملIn Vitro Incorporation of Uridine-h3 into Developing Fruit Fly Oocytes
The developing egg of Drosophila melanogaster consists of a 16-cell nest surrounded by an envelope of follicle cells. The 16 cells are daughters which arise by four consecutive divisions of an oogonium. Fifteen of the daughter germ cells differentiate into nurse cells and nourish the most posterior daughter germ cell which becomes the oocyte. The nurse cell chromosomes elongate by uncoiling and...
متن کاملThe Drosophila gene fs(2)cup interacts with otu to define a cytoplasmic pathway required for the structure and function of germ-line chromosomes.
The Drosophila ovarian tumor gene (otu) encodes cytoplasmic proteins that are required in germ-line cells for cyst formation, nurse cell chromosome structure and egg maturation. We have analyzed a gene, fs(2)cup, that participates in many of the same processes and interacts with otu genetically. Both nurse cell and oocyte chromosomes require cup to attain a normal morphology. In addition, the g...
متن کاملNucleotide sequence of a cDNA from the putative ovarian tumor locus of Drosophila melanogaster.
A cDNA from the putative ovarian tumor (otu) locus of the Drosophila melanoqaster X-chromosome was isolated from a Canton S ovary library using a genomic probe. The otu gene is essential for female fertility, and produces a variety of ovarian phenotypes when mutant (1). The cDNA corresponds tc an abundant 3.2 kb message present in nurse cells and the developing oocyte (2,3). A 2433 b.p. open re...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Methods in molecular biology
دوره 247 شماره
صفحات -
تاریخ انتشار 2004